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WAO Medical Book Reviews

Posted: July 7, 2016

Mast Cells: Methods and Protocols, Second Edition
Edited by: Michael R. Hughes, Kelly M. McNagny|
ISBN 978-1-4939-1567-5
eISBN: 978-1-4939-1568-2
DOI: 10.1007/978-1-4939-1568-2

$119.00 USD / €103.52 EUR (eBook)
$159.00 USD / € 133.74 EUR (Hardcover)
Available from: Springer

Pavel Kolkhir, MD, PhD
I.M. Sechenov First Moscow State Medical University
Department of Dermatology and Venereology
Moscow, Russia

Mast cells are key effector cells of the immune system. They play a vital role in allergy and anaphylaxis but are also involved in wound healing, angiogenesis, and autoimmunity. The first edition of Mast Cells: Methods and Protocols, the description of essential mast cell techniques, was published in 2006. The present book is considered an extension rather than a replacement for the previous edition. The editors of this volume invited top investigators in mast cell biology to share their ideas and scientific insights into techniques and methods of mast cell isolation, immunobiology and the animal models of human disease.  

A broad selection of reviews and detailed step-by-step instructions for adaptation and designing of experiments provides state-of-the-art knowledge and useful approaches in the field of mast cells for neophytes as well as experienced researchers.

Mast Cells: Methods and Protocols consist of five related parts. Part I is organized into six introductory and fundamental review chapters, which summarize what is currently known on ontology, phylogeny, and tissue distribution of mast cells. Methods for the study of mast cell recruitment and accumulation in tissues are discussed. Part II focuses on the function of mast cells in human health and disease and describes methods of mast cell characterization and isolation from human tissue. In part III, the authors present comprehensive techniques, methods and protocols to study the molecular mechanisms of mast cell function and activation. Part IV covers mast cell products and mediators. Assays for evaluation of exocytosis, signaling processes, degranulation of mast cells and cytokine release are presented. Part V is the largest section of the book and describes murine models of disease for the investigation of mast cell function in autoimmune disorders, cancer, and allergic diseases. The edition includes 64 high-quality illustrations (18 in black and white and 46 in color) which facilitate understanding of the described processes.

Assessment and Audience
Several chapters include valuable review topics that give a comprehensive overview of mast cell development, function and their causal role in different diseases. Cutting-edge, easily reproducible protocols and methods for the isolation of mast cells and investigation of the molecular mechanisms of their activation and functions can help investigators plan and carry out their experiments. This book is aimed primarily at laboratory researchers but also may be useful for all specialists who are interested in mast cell biology.

Why read this book?
The book is well written, illustrated, summarizes extensive recommendations of world-renowned experts in the field and offers an up-to-date and in-depth look into mast cell biology.